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- W2355749214 abstract "Objective To construct several eukaryotic plasmid expressing shRNA of gene bi-1 which derived from the eukaryotic plasmid pmU6 and study the effect on proliferation of CNE-2Z, CNE-1, HO8910, HO8910PM.Methods Oligonucleotides of bi-1 were synthesized and inserted into the eukaryotic expression vector pmU6 in definite direction. Cells were transfected with recombinant plasmids, MTT assay was applied to evaluate proliferation of cells affected by LipofectmineTM2000 and the plasmids.Results Compared with untreated group, transfected with recombinant plasmids group may inhibit proliferation of CNE-2Z, CNE-1, HO8910PM markedly, but can not inhibit proliferation of HO8910.Conclusion The shRNA expressed by the recombinant plasmid can sufficiently induced RNAi and inbibit cell proliferateion in some tumor cells, and these observations may open a path toward the use of plasmid-mediated RNAi as a new tool for gene therapy research in human cancer." @default.
- W2355749214 created "2016-06-24" @default.
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- W2355749214 date "2008-01-01" @default.
- W2355749214 modified "2023-09-24" @default.
- W2355749214 title "Effect of Eukaryotic Plasmid Expressing shRNA of Gene bi-1 on Proliferation of Human Nasopharyngeal and Ovarian Carcinoma Cell" @default.
- W2355749214 hasPublicationYear "2008" @default.
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