Matches in SemOpenAlex for { <https://semopenalex.org/work/W2355772570> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2355772570 abstract "Objective:To obtain recombinant Epstein Barr virus expression vector pEBAF with N ras cDNA targeted to hepatocelluar carcinoma.Methods:EB virus vector pEBAF which contains human α fetoprotein promoter and enhancer was used as an expression vector.For N ras,the 1.06 kb fragment(including coding region and flanking sequence)from the plasmid pZIP NeoSV(X)1 was excised using BamHI and ligated into the BamHI site of pEBAF treated by phosphorylase in sense and antisense directions.First,poritive clones were selected with restriction map and in situ hybridization.Then DNA sequencing was used for the analysis of its homeogenesis with other related N ras mRNA sequences.Lastly the insert direction of N ras was proved using restriction endonuclease PvuⅡ.Results:The recombinant pEBAF/s N ras and pEBAF/as N ras were both successfully obtained with the molecular biological technique.Conclusion:The two kinds of recombinant pEBAF/N ras expression vectors may lead to targeted expression of N ras on hepatoma and have tissue specificity and exclusiveness for hepatoma.Consequently this will lay foundation for the study of development mechanism and gene therapy of PHC." @default.
- W2355772570 created "2016-06-24" @default.
- W2355772570 creator A5057274151 @default.
- W2355772570 date "2001-01-01" @default.
- W2355772570 modified "2023-09-24" @default.
- W2355772570 title "CONSTRUCTION OF pEBAF EXPRESSION VECTORS OF N RAS SENSE AND ANTISENSE TARGETED TO HEPATOCELLULAR CARCINOMA" @default.
- W2355772570 hasPublicationYear "2001" @default.
- W2355772570 type Work @default.
- W2355772570 sameAs 2355772570 @default.
- W2355772570 citedByCount "0" @default.
- W2355772570 crossrefType "journal-article" @default.
- W2355772570 hasAuthorship W2355772570A5057274151 @default.
- W2355772570 hasConcept C104317684 @default.
- W2355772570 hasConcept C128319531 @default.
- W2355772570 hasConcept C143141573 @default.
- W2355772570 hasConcept C147789679 @default.
- W2355772570 hasConcept C153911025 @default.
- W2355772570 hasConcept C159047783 @default.
- W2355772570 hasConcept C185592680 @default.
- W2355772570 hasConcept C187882448 @default.
- W2355772570 hasConcept C22744801 @default.
- W2355772570 hasConcept C33161422 @default.
- W2355772570 hasConcept C40767141 @default.
- W2355772570 hasConcept C42174275 @default.
- W2355772570 hasConcept C54355233 @default.
- W2355772570 hasConcept C58301748 @default.
- W2355772570 hasConcept C86803240 @default.
- W2355772570 hasConcept C91779695 @default.
- W2355772570 hasConcept C92087593 @default.
- W2355772570 hasConceptScore W2355772570C104317684 @default.
- W2355772570 hasConceptScore W2355772570C128319531 @default.
- W2355772570 hasConceptScore W2355772570C143141573 @default.
- W2355772570 hasConceptScore W2355772570C147789679 @default.
- W2355772570 hasConceptScore W2355772570C153911025 @default.
- W2355772570 hasConceptScore W2355772570C159047783 @default.
- W2355772570 hasConceptScore W2355772570C185592680 @default.
- W2355772570 hasConceptScore W2355772570C187882448 @default.
- W2355772570 hasConceptScore W2355772570C22744801 @default.
- W2355772570 hasConceptScore W2355772570C33161422 @default.
- W2355772570 hasConceptScore W2355772570C40767141 @default.
- W2355772570 hasConceptScore W2355772570C42174275 @default.
- W2355772570 hasConceptScore W2355772570C54355233 @default.
- W2355772570 hasConceptScore W2355772570C58301748 @default.
- W2355772570 hasConceptScore W2355772570C86803240 @default.
- W2355772570 hasConceptScore W2355772570C91779695 @default.
- W2355772570 hasConceptScore W2355772570C92087593 @default.
- W2355772570 hasLocation W23557725701 @default.
- W2355772570 hasOpenAccess W2355772570 @default.
- W2355772570 hasPrimaryLocation W23557725701 @default.
- W2355772570 hasRelatedWork W1984175957 @default.
- W2355772570 hasRelatedWork W2019886119 @default.
- W2355772570 hasRelatedWork W2350404005 @default.
- W2355772570 hasRelatedWork W2353828860 @default.
- W2355772570 hasRelatedWork W2356774747 @default.
- W2355772570 hasRelatedWork W2360025459 @default.
- W2355772570 hasRelatedWork W2362247757 @default.
- W2355772570 hasRelatedWork W2365193215 @default.
- W2355772570 hasRelatedWork W2369122809 @default.
- W2355772570 hasRelatedWork W2373437744 @default.
- W2355772570 hasRelatedWork W2377989026 @default.
- W2355772570 hasRelatedWork W2379266697 @default.
- W2355772570 hasRelatedWork W2381343262 @default.
- W2355772570 hasRelatedWork W2381683784 @default.
- W2355772570 hasRelatedWork W2382801406 @default.
- W2355772570 hasRelatedWork W2391070020 @default.
- W2355772570 hasRelatedWork W2403486817 @default.
- W2355772570 hasRelatedWork W2135702454 @default.
- W2355772570 hasRelatedWork W2156853466 @default.
- W2355772570 hasRelatedWork W2205860907 @default.
- W2355772570 isParatext "false" @default.
- W2355772570 isRetracted "false" @default.
- W2355772570 magId "2355772570" @default.
- W2355772570 workType "article" @default.