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- W2355802153 abstract "Objective To prepare and verify the HLA-A*2402 tetramer loaded with melanoma associated antigen gp100 peptide.Methods HLA-A*2402-BSP,i.e.the ectodomain of HLA-A*2402 fused to a BirA substrate peptide(BSP) was refolded in the presence of human β2-microglobulin as well as the gp100 peptide(VYFFLPDHL,VYF).The biotinylated HLA-A*2402/VYF molecules were purified,then bound to streptavidin-PE and tetramerized.The extent of tetramerization was detected by SDS-PADE under non-reducing conditions without boiling the sample.Finally,the prepared HLA-A*2402/VYF tetramer reagent was verified by detecting the CD8+ T cells specific for gp100 in HLA-A*2402 positive healthy volunteers by three-color flow cytometry.Results HLA-A*2402/VYF tetramer was successfully prepared.The extent of tetramerization was about 83%,and the tetramer positive rates were 0.02% to 0.06% within the CD8+ T cell populations in the peripheral blood of healthy HLA-A*2402 positive healthy volunteers.Conclusion The regent will facilitate the study of the immune responses of gp100 VYF-specific CD8+ T cells in the clinical trials of immunotherapy on melanoma patients with HLA-A*2402 allele." @default.
- W2355802153 created "2016-06-24" @default.
- W2355802153 creator A5028926069 @default.
- W2355802153 date "2010-01-01" @default.
- W2355802153 modified "2023-09-23" @default.
- W2355802153 title "Preparation and verification of HLA-A~*2402 tetramer loaded with melanoma associated antigen gp100 peptide" @default.
- W2355802153 hasPublicationYear "2010" @default.
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