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- W2355828880 abstract "The ultraviolet-visible spectrophotometry(UV) was used to determine total flavonoids in Equisetum pratense Ehr.at the detection wavelength of 508 nm.The high-performance liquid chromatography(HPLC) was use to determine the kaempferol.The chromatographic column was Hypersil C18 column(4.6 mm×250 mm,5 μm),the mobile phase was methanol-0.4% phosphoric acid(56∶44) at the flow rate of 1.0 mL·min-1,the detection wavelength was 365 nm and column temperature was ambient temperature.The results indicated that the linear relation of rutin was good in the range of 0.01~0.06 mg/mL,the regression equation was A=11.475C+0.009 6(r=0.998 9),the average recovery rate was 101.87%,and RSD was 1.57%.The linear relation of kaempferol was good in the range of 0.057~1.140 μg,regression equation was Y=4 636.2X-47 562(r=0.999 9),the average recovery was 99.03% and RSD was 1.60%.This method is simple,sensitive and accurate in operation and good in repeat.It is suitable for the determination of total flavonoids and kaempferol in E.pratense Ehr." @default.
- W2355828880 created "2016-06-24" @default.
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- W2355828880 date "2011-01-01" @default.
- W2355828880 modified "2023-09-25" @default.
- W2355828880 title "Determination of Contents of Total Favonoids and Kaempferol in Equisetum pratense by UV and HPLC" @default.
- W2355828880 hasPublicationYear "2011" @default.
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