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- W2355881310 abstract "Objective To Investigate the antitumor immunity of the EWS FLI1 gene and hGM CSF gene modified dendritic cells (DC) in vitro.Methods With the hGM CSF adenovirus (AdhGM CSF) and 1 000 U/ml IL 4,the DCs from peripheral mononuclear cells were cultured and analyzed by fluorescence activated cell sorting (FACS) and mixed lymphocyte reaction (MLR).Following lipofectamine mediated transfection to hGM CSF gene modified DC with expressing plasmid pCA13/EWS FLI1 encoding the EWS FLI1 gene,the expression of chimeric gene EWS FLI1 was detected by RT PCR.The antigen specific CTL was induced by DCs modified with the EWS FLI1 gene and hGM CSF gene.A LDH release assay was used to analyze the antigen specific CTL response against A673 cells.Results The DCs modified with hGM CSF gene showed the typical shape,high level expression of CD83,HLA DR,CD80,CD86 and acted as a potent immune stimulator to allogenic lymphocyte,which was similar to traditionally cultured DC.After the DC were transfected with pCA13/EWS FLI1,the EWS FLI1 mRNA was easily detected by RT PCR.The antigen specific CTL was induced successfully by the EWS FLI1 gene and hGM CSF gene modified DC.The vigorous antigen specific CTL response against A673 cells was detected by LDH release assay.The percent killing was (29.950?0±0.011?7)% at effector target ratio 20∶1,which was more efficient than that of the control.Conclusion The chimeric gene of Ewing sarcoma EWS FLI1 can be expressed in the DCs modified with hGM CSF gene after T lymphocytes were stimulated by DCs modified with EWS FLI1 gene and hGM CSF gene.The vigorous antigen specific CTL activity against A673 cells can be detected." @default.
- W2355881310 created "2016-06-24" @default.
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- W2355881310 date "2004-01-01" @default.
- W2355881310 modified "2023-09-25" @default.
- W2355881310 title "In vitro study of anti tumor immunity of the EWS-FLI1 gene and hGM CSF gene modified dendritic cells" @default.
- W2355881310 hasPublicationYear "2004" @default.
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