Matches in SemOpenAlex for { <https://semopenalex.org/work/W2355885439> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2355885439 abstract "Objective Construction of an eukaryotic expression plasmid of E gene fragment from Dengue 2 virus and eukaryotic expression of E glycosylated protein. Methods The E gene fragment of Dengue 2 virus NGC strain was amplified by RT PCR and this fragment was cloned into eukaryotic expression vector pcDNA3. The recombinant plasmid pcDNA3 E was thus constructed and identified by sequencing and digestion with restriction enzymes. The recombinant plasmid pcDNA3 E was transfected into NIH3T3 cell by lipofectin. The expressed protein was analyzed by immunofluorescence, SDS PAGE and Western blotting assay. Results A recombinant eukaryotic expression plasmid pcDNA3 E was successfully constructed. The recombinant plasmid expressed a 60×10 3 protein of Dengue 2 virus. Conclusions The constructions of eukaryotic expression of E gene fragment from Dengue 2 virus and eukaryotic expression of E glycosylated protein will be helpful to study the structure and functions of the E protein, and to the development of Dengue diagnostic agents as well as Dengue virus nucleic acid vaccine." @default.
- W2355885439 created "2016-06-24" @default.
- W2355885439 creator A5018471110 @default.
- W2355885439 date "2000-01-01" @default.
- W2355885439 modified "2023-09-23" @default.
- W2355885439 title "The expression of E gene region of Dengue 2 virus in NIH3T3 and its identification" @default.
- W2355885439 hasPublicationYear "2000" @default.
- W2355885439 type Work @default.
- W2355885439 sameAs 2355885439 @default.
- W2355885439 citedByCount "0" @default.
- W2355885439 crossrefType "journal-article" @default.
- W2355885439 hasAuthorship W2355885439A5018471110 @default.
- W2355885439 hasConcept C104317684 @default.
- W2355885439 hasConcept C128319531 @default.
- W2355885439 hasConcept C153911025 @default.
- W2355885439 hasConcept C159047783 @default.
- W2355885439 hasConcept C22744801 @default.
- W2355885439 hasConcept C2522874641 @default.
- W2355885439 hasConcept C2779635636 @default.
- W2355885439 hasConcept C33161422 @default.
- W2355885439 hasConcept C40767141 @default.
- W2355885439 hasConcept C533803919 @default.
- W2355885439 hasConcept C54009773 @default.
- W2355885439 hasConcept C54355233 @default.
- W2355885439 hasConcept C86803240 @default.
- W2355885439 hasConcept C92087593 @default.
- W2355885439 hasConceptScore W2355885439C104317684 @default.
- W2355885439 hasConceptScore W2355885439C128319531 @default.
- W2355885439 hasConceptScore W2355885439C153911025 @default.
- W2355885439 hasConceptScore W2355885439C159047783 @default.
- W2355885439 hasConceptScore W2355885439C22744801 @default.
- W2355885439 hasConceptScore W2355885439C2522874641 @default.
- W2355885439 hasConceptScore W2355885439C2779635636 @default.
- W2355885439 hasConceptScore W2355885439C33161422 @default.
- W2355885439 hasConceptScore W2355885439C40767141 @default.
- W2355885439 hasConceptScore W2355885439C533803919 @default.
- W2355885439 hasConceptScore W2355885439C54009773 @default.
- W2355885439 hasConceptScore W2355885439C54355233 @default.
- W2355885439 hasConceptScore W2355885439C86803240 @default.
- W2355885439 hasConceptScore W2355885439C92087593 @default.
- W2355885439 hasLocation W23558854391 @default.
- W2355885439 hasOpenAccess W2355885439 @default.
- W2355885439 hasPrimaryLocation W23558854391 @default.
- W2355885439 hasRelatedWork W2021120869 @default.
- W2355885439 hasRelatedWork W2148594464 @default.
- W2355885439 hasRelatedWork W2350781849 @default.
- W2355885439 hasRelatedWork W2360457679 @default.
- W2355885439 hasRelatedWork W2362255091 @default.
- W2355885439 hasRelatedWork W2365202871 @default.
- W2355885439 hasRelatedWork W2366218191 @default.
- W2355885439 hasRelatedWork W2368365196 @default.
- W2355885439 hasRelatedWork W2370549919 @default.
- W2355885439 hasRelatedWork W2371993280 @default.
- W2355885439 hasRelatedWork W2373428276 @default.
- W2355885439 hasRelatedWork W2376117368 @default.
- W2355885439 hasRelatedWork W2377960174 @default.
- W2355885439 hasRelatedWork W2383732229 @default.
- W2355885439 hasRelatedWork W2391908860 @default.
- W2355885439 hasRelatedWork W3087584240 @default.
- W2355885439 hasRelatedWork W3095161090 @default.
- W2355885439 hasRelatedWork W3116049113 @default.
- W2355885439 hasRelatedWork W3146495747 @default.
- W2355885439 hasRelatedWork W2155450234 @default.
- W2355885439 isParatext "false" @default.
- W2355885439 isRetracted "false" @default.
- W2355885439 magId "2355885439" @default.
- W2355885439 workType "article" @default.