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- W2355896152 abstract "To make a kind of nonpathogenic Bacillus subtilis BS224 with the antibacterial characteristics to express and secret basic fibroblast growth factor (bFGF) so that it has both antibacterial and accelerating wound healing nature. We constructed an integrative vector with the α-amylase promoter and signal sequence coding region obtained by PCR from the chromosome of an α-amylase hyperproducing strain, and the synthesized gnt (gluconate) terminator, and the 3^sequence of α-amylase gene inserting into plasmid pHV32. The bFGF cDNA was inserted into the vector. BS224 was transformed by protoplast transformation. The transformants were screened by chloromycetin resistance and they were verified to integrate at the amy locus of BS224 by α-amylase secretion analysis and Southern hybridization. Basic fibrobast growth factor expression was tested by Western immunoblot analysis." @default.
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- W2355896152 date "2002-01-01" @default.
- W2355896152 modified "2023-09-24" @default.
- W2355896152 title "Basic fibroblast growth factor expressed in Bacillus subtilis BS224" @default.
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