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- W2355901473 abstract "Objective To study the effect of puma gene on the apoptosis of HeLa cell in vitro and whether the protein of cytochrome c(Cyt c) and apoptosis inducing factor(AIF) in the chondrosome participate in the process of apoptosis.MethodsLiposome HeLa cell was transfected and the result of transfection was assessed by RT-PCR,then the morphologic changes of HeLa cell induced by puma gene were detected by AO/EB dyeing.Finally the position of Cyt c and AIF transferred in the chondrosome was studied by Western blot.ResultsAO/EB dyeing displayed that HeLa cell had a series of apoptosis morphologic changes after transfecting puma into HeLa cell,and the changes after 48h were more obvious compared to 24h.Western blot displayed that the protein Cyt c and AIF transferred toward to kytoplasm after the expression of puma gene.The mount of transfected protein after 48h was more obvious compared to 24h.ConclusionPuma gene has the effect of promo-ting apoptosis of HeLa cell in which Cyt c and AIF take part." @default.
- W2355901473 created "2016-06-24" @default.
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- W2355901473 date "2008-01-01" @default.
- W2355901473 modified "2023-09-24" @default.
- W2355901473 title "Effect and mechanism of puma gene on the apoptosis of HeLa cell in vitro" @default.
- W2355901473 hasPublicationYear "2008" @default.
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