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- W2355926350 abstract "Objective To investigate the antioxygenation and protective effect of quercetin polydatin and genistein on HaCaT cells injured by UVB irradiation.Methods The antioxygenation of quercetin polydatin and genistein compared on the base of the elimination tests of DPPH free radical and hydreoxide(·OH) free radical with vitamin C as positive control,and HaCaT cells were incubated in the medium supplemented with quercetin polydatin and genistein 100 μmol / L for 30 min before irradiating HaCaT cells with UVB(30 mJ / cm2).Irradiated by UVB for 12 h,the cells were collected,and the viability of cell proliferation was detected by MTT method.The level of TNF-α was detected by ELISA method,the content of MDA and the activity of SOD were detected by reagent kit.Results Both of them above could scavenge DPPH and·OH free radical.The scavenging ability was genistein polydatin quercetin.UVB irradiation caused significant damage to HaCaT cells.However,when the three drugs acted on HaCaT cells,compared with those treated by UVB irradiation only,the viability and the activity of SOD of HaCaT cells increased(P 0.05),accompanied by a decrease of TNF-α and MDA level(P 0.05).Conclusion Those three drugs can inhibit UVB irradiation-induced HaCaT cells damage,promote cell survival proliferation,and have a protection effect on HaCaT cells injured by UVB irradiation.Maybe one of mechanisms related to its inhibition to the damage effect of UVB irradiation on HaCaT cells is clearing free radicals and enhancing the antioxidant capacity of cells." @default.
- W2355926350 created "2016-06-24" @default.
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- W2355926350 date "2013-01-01" @default.
- W2355926350 modified "2023-09-23" @default.
- W2355926350 title "Study on antioxidant activities of quercetin,polydain and genisten and the protection on HaCa T cells against damage from UVB irradiation" @default.
- W2355926350 hasPublicationYear "2013" @default.
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