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- W2355937229 abstract "Objective To extract and purify polysaccharides (LP)from shell of Dimocarpus longan Lour.,measure the polysaccharide contents in shell of Dimocarpus longan Lour. and investigate LP immune function in vitro. Method Crude polysaccharide was isolated by adding EtOH to the hot water extracts of Dimocarpus longan Lour. and was deproteinized by Sevage method and emzymic method,and were decolourised by H2O2. Phenol-sulfuric acid method was employed in the determination of total sugar and polysaccharide contents in shell of Dimocarpus longan Lour. The crude polysaccharide was further purified by DEAE-cellulose (OH-) ion-exchange and Sephadex G-100 chromatography. ConA-induced splenic lymphocyte proliferation and activity of secretion of IL-2 in mice were determined respectively by 3-(4,5-2dimethylthiazal-2yl)2,5-diphenyltetrazoliumbromide(MTT) assay and enzyme-linked immunosorbent assay (ELISA). Results The total content of sugar and the content of polysaccharides LP were 51.84% and 3.22%,respectively,and the content of LP were 92.01% after purification.The ConA-induced spleen lymphocyte proliferation and the ConA-induced secretion of IL-2 in the different doses of LP were decreased and enhanced in dose-dependent manner. Conclusion LP is able to enhance immune function of spleen lymphocytes." @default.
- W2355937229 created "2016-06-24" @default.
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- W2355937229 date "2010-01-01" @default.
- W2355937229 modified "2023-09-26" @default.
- W2355937229 title "Measurement of the polysaccharide contents in shell of Dimocarpus longan Lour. and evaluation its immune ability in vitro" @default.
- W2355937229 hasPublicationYear "2010" @default.
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