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- W2355984961 abstract "A population of transgenic plants was produced by the transformation of internodal explants of potato using an Agrobacterium tumefaciens LBA4404-based vector containing a gomphosis gene(PSAG12-ipt).The results showed that intermodal explants were more effective for transformation than leaf explants.The regeneration strategy utilised a three-step protocol,a 2 days pre-culture on the MS medium containing 6-benzylaminopurine(6-BA) 0.25 mg/L,α-naphythy lacetic acid(NAA) 0.25 mg/L,2,4-dichlorophenacetic acid(2,4-D) 0.25 mg/L,and supplemented 1% Na2SO3,followed the explants of pre-culture were incubated 8 min in Agrobacterium tumefaciens LBA4404 suspension(OD600 0.2~0.5),last,the explants were co-cultured 3 days on basal medium without supplemented any phytohormones.After 3 days co-cultivation,the explants of internode and leaf were transferred to basal medium supplemented phytohormones,1% Na2SO3,200 mg/L cefotaxime and 75 mg/L kanamycin until to regenerated plants.Transgenic plants were identified utilizing PSAG12 and ipt gene dual primer by PCR.The positive transformation rate was 65.8%.Southern blotting analysis identification showed that most ipt gene were induced into potato genome only one copy." @default.
- W2355984961 created "2016-06-24" @default.
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- W2355984961 date "2012-01-01" @default.
- W2355984961 modified "2023-09-24" @default.
- W2355984961 title "Transformation of P_(SAG12)-ipt Gomphosis Gene into Potato" @default.
- W2355984961 hasPublicationYear "2012" @default.
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