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- W2355992484 abstract "A Dot-PPA-ELISA was developed for the detection of chlamydial antibody in porcine sera.A total of 120 swine serum samples were tested.The results showed that Dot-PPA-ELISA detected 35.83%(43 of 120)positive with an antibody titer of 1:256 or higher.In comparison,Indirect hemagglutination test(IHA)only detected 21.67%(26 of 120)positive with a titre of 1:64 or higher.The Dot-PPA-ELISA assay was highly specific and did not react with positive sera of swine fever,swine brucellosis,swine foot and mouth disease and swine bow-shaped body disease.It has a high sensitivity of detecting as little as 1.21 ng swine chlamydial IgG.The assay was highly reproducible(94.44%)and was not affected by the quality of microtiter plate.The chlamydial antigen conjugated NC membranes could be stored at-20℃,4℃or room temperature for at least three months and remain as sensitive.This method was simple,cost effective and could be performed at room temperature in about 3 hours.The Dot-PPA-ELISA could also be used for other mammal because PPA is a broad-spectrum diagnostic reagent." @default.
- W2355992484 created "2016-06-24" @default.
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- W2355992484 date "2007-01-01" @default.
- W2355992484 modified "2023-09-25" @default.
- W2355992484 title "Detection of chlamydial antibody in porcine sera by dot horseradish peroxidase enzyme-linked straphylococcus protein a immnosorbent assay(Dot-PPA-ELISA)" @default.
- W2355992484 hasPublicationYear "2007" @default.
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