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- W2355994997 abstract "Objective: To explore the method for induction and identification of tetraploid Codonopsis pilosula,therefore setting up the basis on the breeding of new cultivars with high-quality and high resistance to biotic or abiotic stress.Methods: Seeds of C.pilosula were treated with colchicine.After germination, hypocotyls were selected for culture,and then the regenerated plants were screened by counting the number of chromosomes to find tetraploid strains.Results: The optimal concentration of colchicine for induction is 0.02% ; the optimal solution for dissociation of cells in root ti Pis a mixture of hydrochloric acid and acetic acid( 45% ) with a radio of 2∶1,and the optimal dissociation time is 20-30 s; the optimal sampling time for chromosome counting in a day depends on the month for sample collection; combination of direct and indirect identification technologies can ensure the accuracy of identification and reduce the redundancy of task.Conclusion: The technologies for induction and identification of tetraploid C.pilosula will play a key role in breeding of new cultivars with ploidy stability and high-quality." @default.
- W2355994997 created "2016-06-24" @default.
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- W2355994997 date "2013-01-01" @default.
- W2355994997 modified "2023-09-28" @default.
- W2355994997 title "Induction and Identification of Tetraploid Codonopsis pilosula( Franch. ) Nannf." @default.
- W2355994997 hasPublicationYear "2013" @default.
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