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- W2356108356 abstract "Modified magnetic chitosan microspheres(MMCM) were prepared with reversed-phase suspension polymerization method using HEMA and GMA as monomers and ammonium persulfate as initiator.Lactase was immobilized on MMCM via adsorption,covalent bonding and cross-linking with glutaraldehyde.The optimum conditions of lactase immobilization were: MMCM were previously swelled in 0.1 M pH 7.0 phosphate buffer sufficiently,lactase was added to 2.0 U per mg of carrier and incubated at 4 ℃ for 3 h,reacted with 0.1% glutaraldehyde for 4 h.The activity of immobilized lactase was 685.14 U/g and the activity recovery was 34.3%.In comparison with the free enzyme,the pH and thermal stability of immobilized lactase were increased.After continuous operation for 10 cycles,the immobilized lactase activity was remained over 70%." @default.
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- W2356108356 date "2011-01-01" @default.
- W2356108356 modified "2023-09-26" @default.
- W2356108356 title "Immobilization of lactase on modified magnetic chitosan microspheres" @default.
- W2356108356 hasPublicationYear "2011" @default.
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