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- W2356116162 abstract "Objective:To investigate the reference range of MPLI(Mycoplasma pneumoniae load index) and its usefulness in the diagnosis of Mycoplasma pneumoniae(MP) infection in children.Methods:128 throat swabs were collected from 128 healthy children aged between 3 and 6 years at the end of March 2008.Fluorescence quantitative PCR(FQ-PCR) assay was used to analyze MP-DNA and human actin-DNA(H actin-DNA) for those swabs.MPLI was then calculated: MPLI=-lg(MP-DNA copies/H actin-DNA copies),followed by the calculation of its reference rage(P5).110 throat swabs were collected with paired sera from the department of pediatry in our hospital from March to July 2008.The former FQ-PCR assay was used to analyze throat swabs,MP-IgM assay for the sera.It was made a gold standard for MP infection that MP-IgM was positive once or twice,on which sensitivity(SEN),specificity(SPE),positive predictive value(+PV),negative predictive value(-PV) and receiver operator characteristic curve(ROC) for MPLI were made based.P5 was compared with the cut-off from ROC.Results:The cut-off from ROC was better than that from P5 of 128 normal samples.Thus,the reference range of MPLI was determined as above 5.90 for children aged 3 to 6 years.26 children were diagnosed MP infection among those 110 samples according to the gold standard.Then the values of SEN,SPE,+PV,-PV were 73.1%,100.0%,100.0% and 92.3% for a single MP-IgM,all 100.0% for double MP-IgM respectively.Such were 26.9%,95.2%,63.6% and 80.8% for FQ-PCR alone,26.9%,98.8%,87.5% and 81.4% for MPLI respectively.Conclusion:(1) FQ-PCR assay alone for MP-DNA is limited for its low SEN and high false positive rate,mainly due to non-standarization of clinical samples,which well restricts its clinical large-scale application.(2) The advantage of MPLI lies in its standarization of clinical samples,which makes up the limitation of high false positive rate for FQ-PCR alone and makes the results more persuasive.MPLI could help diagnose acute MP infection earlier than MP-IgM.(3) Under possible conditions(such as economy),MPLI and MP-DNA will be a good combination: it can not only detect acute MP infection earlier,but also help diagnose(early) convalescent state of MP infection." @default.
- W2356116162 created "2016-06-24" @default.
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- W2356116162 date "2009-01-01" @default.
- W2356116162 modified "2023-09-23" @default.
- W2356116162 title "Study on semi-quantitation of Mycoplasma pneumoniae by fluorescence quantitative PCR" @default.
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