Matches in SemOpenAlex for { <https://semopenalex.org/work/W2356161566> ?p ?o ?g. }
Showing items 1 to 44 of
44
with 100 items per page.
- W2356161566 abstract "AIM: To investigate the method of inducing G1 phase synchronization in human endometrial cancer JEC Cells by lovastatin and the cell cycle progress of JEC cells after desynchronization.METHODS: The doubling time of JEC cells was detected by Cell Counting Kit-8(CCK-8) assay.To determine the best lovastatin concentrations for G1 synchronization,JEC cells were treated with lovastatin at concentrations of 10,20,30 and 40 μmol/L for 1× doubling time,and the cell cycle was detected using flow cytometry(FCM).To determine the best period of lovastatin treatment to achieve G1 synchronization,JEC cells were treated with lovastatin at the best concentration for 0.5× to 2× doubling time,and the cell cycle was detected every 4 h using FCM.Furthermore,the cell cycle progress of JEC cells after desynchronization was also observed.RESULTS: The doubling time of JEC cells was almost 24 h.Treatment with lovastatin at the concentration of 20 μmol/L for 24 h achieved maximum G1 arrest [(85.00±0.79)%]in JEC cells.Minimum G1 phase [(35.93±0.44)%]and maximum S phase [(45.28±0.53)%] were observed after desynchronization for 16 h.CONCLUSION: Maximum G1 synchronization of JEC cells is induced by lovastatin at the concentration of 20 μmol/L for 24 h.The JEC cells show minimum G1 phase and maximum S phase after desynchronization for 16 h." @default.
- W2356161566 created "2016-06-24" @default.
- W2356161566 creator A5081219556 @default.
- W2356161566 date "2012-01-01" @default.
- W2356161566 modified "2023-09-23" @default.
- W2356161566 title "Lovastatin-induced G_1 phase synchronization in human endometrial cancer JEC cells" @default.
- W2356161566 hasPublicationYear "2012" @default.
- W2356161566 type Work @default.
- W2356161566 sameAs 2356161566 @default.
- W2356161566 citedByCount "0" @default.
- W2356161566 crossrefType "journal-article" @default.
- W2356161566 hasAuthorship W2356161566A5081219556 @default.
- W2356161566 hasConcept C1491633281 @default.
- W2356161566 hasConcept C153911025 @default.
- W2356161566 hasConcept C185592680 @default.
- W2356161566 hasConcept C2778163477 @default.
- W2356161566 hasConcept C2780940807 @default.
- W2356161566 hasConcept C29537977 @default.
- W2356161566 hasConcept C48900799 @default.
- W2356161566 hasConcept C553184892 @default.
- W2356161566 hasConcept C55493867 @default.
- W2356161566 hasConcept C86803240 @default.
- W2356161566 hasConcept C88254577 @default.
- W2356161566 hasConceptScore W2356161566C1491633281 @default.
- W2356161566 hasConceptScore W2356161566C153911025 @default.
- W2356161566 hasConceptScore W2356161566C185592680 @default.
- W2356161566 hasConceptScore W2356161566C2778163477 @default.
- W2356161566 hasConceptScore W2356161566C2780940807 @default.
- W2356161566 hasConceptScore W2356161566C29537977 @default.
- W2356161566 hasConceptScore W2356161566C48900799 @default.
- W2356161566 hasConceptScore W2356161566C553184892 @default.
- W2356161566 hasConceptScore W2356161566C55493867 @default.
- W2356161566 hasConceptScore W2356161566C86803240 @default.
- W2356161566 hasConceptScore W2356161566C88254577 @default.
- W2356161566 hasLocation W23561615661 @default.
- W2356161566 hasOpenAccess W2356161566 @default.
- W2356161566 hasPrimaryLocation W23561615661 @default.
- W2356161566 hasRelatedWork W1973115127 @default.
- W2356161566 hasRelatedWork W2060926625 @default.
- W2356161566 hasRelatedWork W2395208837 @default.
- W2356161566 isParatext "false" @default.
- W2356161566 isRetracted "false" @default.
- W2356161566 magId "2356161566" @default.
- W2356161566 workType "article" @default.