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- W2356193482 abstract "AIM: To construct the sense and antisense eukaryotic expressing vector of human telomerase reverse transcriptase (hTERT) and then test its expression in human embryonic fibroblast (hEFs). METHODS: cDNA of hTERT in plasmid of pGRN 145 was inserted into eukaryotic fluorescent expressing vector pIRES2 EGFP in sense orientation by DNA recombinant technology and further transfected into human embryonic fibroblast with lipofectin. Expression of hTERT in transfectants was examined by laser confocal microscopy, RT PCR and Western blot. RESULTS: After digestion with Not I, the sense recombinant DNA was digested into two fragments (1.4 kb, 7.4 kb), whereas the antisense recombinant DNA was digested into another two fragments (4.8 kb, 4 .0 kb), which matches the theoretical calculation. The recombinant was transferred into human embryonic fibroblasts. After being selected by G418, one clone was obtained. mRNA and protein of exogenous hTERT gene could be detected by RT PCR and Western blot respectively. CONCLUSION: The sense eukaryotic expressing vector of hTERT was constructed successfully and could be expressed in human embryonic fibroblasts." @default.
- W2356193482 created "2016-06-24" @default.
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- W2356193482 date "2003-01-01" @default.
- W2356193482 modified "2023-09-23" @default.
- W2356193482 title "Construction and expression of eukaryotic fluorescent expressing vector of human telomerase reverse transcriptase in human embryonic fibroblasts" @default.
- W2356193482 hasPublicationYear "2003" @default.
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