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- W2356240857 abstract "Objective To study the resistance to aminoglycosides (AGs) in Escherichia coli which produce extended spectrum β-lactamases (ESBLs),and analyze the relationships among ESBLs,phenotypes of aminoglycosides resistance and aminoglycosides modifying enzymes (AMEs) genes. Methods The ESBLs-producing strains were detected by confirmatory test from 75 strains E.coli; six kinds of AGs were detected by disk diffusion and the genotypes of AMEs were determined by PCR. Results In 75 strains of E.coli,37 (49.33%) ESBLs-producing strains were confirmed. The resistance to AGs in ESBLs-producing strains:gentamicin (78.38%),streptomycin (70.27%),kanamycin (62.16%),tobramycin (50.05%),netilmicin (18.92%),amikacin (10.81%). compared with non-ESBLs-producing strains,there were obvious differences in these aminoglycosides except netilmicin and amikacin and also in the modes of resistance to three and more kinds of AGs (P 0.05). Five kinds of AMEs genes were detected in ESBLs-producing strains and more were aac (3)-Ⅱ(64.86%) and aac (6')-Ⅰ(45.95%); aac(6')-Ⅱ wasn't detected. Compared with non-ESBLs-producing strains,the positive rates were obviously different in aac (3)-Ⅱ,aac (6')-Ⅰ,ant (3 )-Ⅰand double genes'modes (P 0.05). There were correlations between aminoglycosides-resistant phenotypes and genes. Conclusions E.coli resistance to AGs became more serious because of ESBLs producing. There maybe some relationships between ESBLs and AGs in resistance of E.coli." @default.
- W2356240857 created "2016-06-24" @default.
- W2356240857 creator A5081276951 @default.
- W2356240857 date "2010-01-01" @default.
- W2356240857 modified "2023-09-24" @default.
- W2356240857 title "Study on aminoglycosides resistance of extended spectrum β-lactamases-producing strains of Escherichia coli" @default.
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