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- W2356274345 abstract "Objective:To introduce a modified isolation method for acquiring rat atrial myocytes.Methods:The heart of adult sprague-dawley(SD) rat was rapidly excised after successful anesthesia and mounted on a Langendorff apparatus.It was perfused retrogradely via the aorta for five minutes with Ca2+-free Tyrode's solution and then perfused with collagenase dissolved in Tyrode's solution for ten to twelve minutes,and then the atrial appendage was sheared and stored in a kraftbruhe(KB) solution.Thereafter tissue was minced and filtrated.After being centrifuged at 500 r for 2 min,the isolated myocytes were performed gradient calcium reintroduction and allowed to pellet via gravity for 1 hour in refrigerator,then the transient outward potassium current,sodium current and L-tpye calium current were recorded by the use of patch clamp whole cell recording technique.Results:The survival rate of atrial myocytes was high and the morphous and construction of atrial myocytes were fine and the property of calcium-tolerance was good.The transient outward potassium current,sodium current and L-tpye calium current were recorded successfully.Conclusion:The modified isolation method can produce large numbers of single calcium-tolerance myocardial cells and normal electrophysiological properties." @default.
- W2356274345 created "2016-06-24" @default.
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- W2356274345 date "2011-01-01" @default.
- W2356274345 modified "2023-09-23" @default.
- W2356274345 title "A modified method for isolation of rat cardiomyocytes and recording of potassium,sodium and calium current" @default.
- W2356274345 hasPublicationYear "2011" @default.
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