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- W2356282145 abstract "Objective To investigate the effect of nicotine on the mode of human umbilical vein endothelial cells(HUVECs) death.Methods CD34 were used as a marker to identify HUVECs by immunocytochemistry.HUVECs were treated with three different concentrations of nicotine(3,30 and 300 ng/ml) for 24 hours.The rate of apoptosis or necrosis was analyzed with Flow cytometry.Results A low concentration of nicotine(3 ng/ml) was found to promote cell apoptosis.The percentage of apoptosis was significantly decreased when the concentration of nicotine was increased(P0.05).The reduction of apoptosis was accompanied with an increased in necrosis.The increase in necrosis was positively correlated with an increasing concentration of nicotine(r=0.675).Conclusion Nicotine dose-dependently increases necrosis of human umbilical vein endothelial cells." @default.
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- W2356282145 date "2012-01-01" @default.
- W2356282145 modified "2023-09-26" @default.
- W2356282145 title "Effect of nicotine on human umbilical vein endothelial cells" @default.
- W2356282145 hasPublicationYear "2012" @default.
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