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- W2356301930 abstract "Objective To express the non-structral protein 1(NS1)genes of Japanese encephalitis virus,and study the antigenicity and establish the basis for developing the diagnosis kit.Methods The NS1 cDNA was amplified with RT-PCR and cloned into the transfer plasmid pFastBac HTa,then the recombianat transfer plasmid pFastBac-NS1 was transformed into DH10Bac competent cells.The recombinant transposition rBacmid-NS1 was obstained by screening of white plaque and was identified by PCR.After the rBacmid-NS1 transfected into TN cells,the recombinant baculovirus rBV-NS1 was harvested.The expressed protein was detected by SDS-PAGE and Western Blot.Results The SDS-PAGE and Western blot showed that the NS1 gene was well expressed,and the expressed product had antigenicity.Conclusions The stable expression of this protein and the analysis of its antigenic specificity provide the foundation to develop the vaacine for Japanese encephalitis virus." @default.
- W2356301930 created "2016-06-24" @default.
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- W2356301930 date "2011-01-01" @default.
- W2356301930 modified "2023-09-23" @default.
- W2356301930 title "Cloning and Expression of the Nonstructural Protein 1 Gene of Japanese Encephalitis Virus in Insect Cell" @default.
- W2356301930 hasPublicationYear "2011" @default.
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