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- W2356320825 abstract "Objective To explore the feasibility and optimize the working parameters of self-designed endothelial progenitor cells release system(EPCs-RS) when releasing endothelial progenitor cells(EPCs) in vitro.Materials and Methods EPCs were isolated and cultured from 40 ml human peripheral blood.EPCs were released with the EPCs-RS(Patent No.ZL2006200406232) under a different series of working pressure and releasing speed,then the cell survival rates were tested by Annexin V and PI stain.Results The EPCs-RS can release EPCs effectively.A series data demonstrated there was no variation of cell survival rate before and after releasing cells under a pressure less than 3 atm and a speed less than 0.3 ml/s.It also showed that EPCs could remain with the highest activity when released at the pressure of 2.5 atm and the releasing speed of 0.2 ml/s.Conclusion Under certain working pressure and releasing rate,the EPCs-RS can release EPCs effectively.This system may have been a novel device and provided a new method in treating peripheral vascular occlusive disease and preventing restenosis after PTA therapy." @default.
- W2356320825 created "2016-06-24" @default.
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- W2356320825 date "2009-01-01" @default.
- W2356320825 modified "2023-09-28" @default.
- W2356320825 title "Design and in Vitro Study of an Endothelial Progenitor Release System" @default.
- W2356320825 hasPublicationYear "2009" @default.
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