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- W2356337118 abstract "Objective:To explore the methods of preparing leukemia mouse model which were prepared with SPF Balb/c mouse irradiated by X ray and K562 cells that were transfered GFP and NeoR gene.Methods:Balb/c mice of experimental group were irradiated 2 or 3Gy,then injected into log phase growth K562(GFP+/Neo+) cells 2×106 by vena caudalis after 24hours,whereas the mice of control group were not treated.Results:The mouse of experimental group died within 30 and 24 days;there was significant difference in survival time compared with that of normal control group(P0.01).The weight decreased significantly than that in normal control group(P0.05).The incidence of leukemia in processed mice was 100%,and had no natural relief.With the increase of irradiation dose,survive time of mice shorten gradually,the percentage of leukemia cells in peripheral blood and bone marrow increased gradually.The existence of GFP and NeoR gene was verified by FCM and PCR in peripheral blood,bone marrow,live and spleen.Conclusion:The leukemia mouse model can be made by infusing K562 cells by vena caudalis into Balb/c mouse after irradiated." @default.
- W2356337118 created "2016-06-24" @default.
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- W2356337118 date "2011-01-01" @default.
- W2356337118 modified "2023-09-22" @default.
- W2356337118 title "Establish ment of leukemia mouse model using Balb/c mouse irradiated by X ray" @default.
- W2356337118 hasPublicationYear "2011" @default.
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