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- W2356337164 abstract "【Objective】 Specific monoclonal antibodies(mAbs) against FMDV O was prepared so as to provide materials for the study of diagnostic method of FMD.【Method】 By fusing SP2/0 myeloma cells and spleen cells of Balb/c mice immunized with purified virus preparations with the standard hybridomas technique,indirect ELISA,indirect immunofluorescence assay(IFA) and limited dilution for cell cloning were performed to screen positive hybridomas,then the biological characteristics of the mAbs were identified.【Result】 Two stable hybridoma cell strains,named 2B9 and 5F7,were established.These mAbs were IgG1/κ isotype and contained kappa light chains.IFA showed that they reacted with FMDV serotype O,but not with FMDV serotype Asia1.Western blot analysis suggested that the epitopes they recognized were conformational since any specific band didn't appear.The additive ELISA showed that antigen epitopes recognized by 2B9 and 5F7 could be close to each other.Their relative affinity index were 1.5 mol/L,which was indicated by thiocyanate elution measurement.Neutralization test showed that they didn't show any neutralizing activity to O serotype FMDV strains.【Conclusion】 These two mAbs could be used as experimental materials for the establishment of the diagnostic method of FMD." @default.
- W2356337164 created "2016-06-24" @default.
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- W2356337164 date "2008-01-01" @default.
- W2356337164 modified "2023-09-24" @default.
- W2356337164 title "Production and characterization of monoclonal antibodies against Foot-and-mouth Disease Virus serotype O" @default.
- W2356337164 hasPublicationYear "2008" @default.
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