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- W2356345233 abstract "For preparation of specific polyclonal antibodies against EGFRvⅢ,animals were immunized with the synthesized EGFRvⅢ specific peptide PEP3,which was coupled with hemocyanin,and the antiserum were raised.The pET30a/E33XI prokaryotic expression-vector was constructed and fusion protein His-E33(a part of extracellular Domain of EGFRvⅢ) was expressed and purified.After preparation of a column for purification of antibodies with the fusion protein E33XI as antigen,the specific polyclonal antibodies against EGFRvⅢ were extracted through the column-purification.Immunohistochemistry with U87,U251,HeLa and HEK-293 cells were performed to verify specificity of the antibodies." @default.
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- W2356345233 date "2009-01-01" @default.
- W2356345233 modified "2023-09-26" @default.
- W2356345233 title "Preparation of Polyclonal Antibody Against Epidermal Growth Factor Receptor Variant III(EGFRvIII) and Its Specificity" @default.
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