Matches in SemOpenAlex for { <https://semopenalex.org/work/W2356349324> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2356349324 endingPage "6" @default.
- W2356349324 startingPage "983" @default.
- W2356349324 abstract "To investigate the expression in Escherichia coli (E. coli) of hepatitis B virus (HBV) genes from minority nationality patients in Yunnan province with chronic hepatitis B (CHB) and their antigenicity.Peripheral blood samples were collected from 25 minority nationality patients with CHB in Yunnan province. Twenty-five CHB patients of Han nationality in Yunnan were used as controls. The full length HBV preS2/S and C genes were amplified by PCR, cloned, sequenced, and inserted into the prokaryotic expression vector p lambda PR. The recombinant plasmids p lambda PR-S2S and p lambda PR-C were transfected into E. coli of the line TOP10. The expression of the non-fusion proteins encoded by the HBV preS2S and C genes was determined by sodium dodecyl sulphate polyacrlamide gel electrophoresis (SDS-PAGE) and Western blotting. The antigenicity of the non-fusion proteins was examined by ELISA. Fifty samples of serum of patients with hepatitis A, 50 samples of serum of patients with hepatitis C, and 50 samples of serum of healthy blood donors were used as controls in the study of the antigenicity of non-fusion proteins.SDS-PAGE showed that the recombinant plasmids p lambda PR-S2S and p lambda PR-C were both stably and highly expressed in the E. coli for all 50 CHB patients. The molecular weights of the expressed non-fusion proteins, with a concentration of 16% and a purity of 50%, were between 31,000 and 21,000. Western blotting and ELISA showed that the purified recombinant non-fusion proteins reacted strongly with the antibodies HBpreS2S/SAb and HBcAb and the serum of CHB patients, but there was no cross-activity between the non-fusion proteins and all the serum samples of controls with HA and HC, and normal controls.The HBV preS2S and C genes from the minority nationality patients with CHB can be stably and highly expression in E. coli. The non-fusion proteins encoded by the HBV preS2S and C genes have high antigenicity. These findings have potential values in development of HB vaccines." @default.
- W2356349324 created "2016-06-24" @default.
- W2356349324 creator A5000483639 @default.
- W2356349324 creator A5000821623 @default.
- W2356349324 creator A5016207436 @default.
- W2356349324 creator A5031023052 @default.
- W2356349324 creator A5060682672 @default.
- W2356349324 date "2006-04-11" @default.
- W2356349324 modified "2023-09-23" @default.
- W2356349324 title "[Expression in Escherichia coli of hepatitis B virus genes from minority nationality patients in Yunnan province with chronic hepatitis B and their antigenicity]." @default.
- W2356349324 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16759541" @default.
- W2356349324 hasPublicationYear "2006" @default.
- W2356349324 type Work @default.
- W2356349324 sameAs 2356349324 @default.
- W2356349324 citedByCount "0" @default.
- W2356349324 crossrefType "journal-article" @default.
- W2356349324 hasAuthorship W2356349324A5000483639 @default.
- W2356349324 hasAuthorship W2356349324A5000821623 @default.
- W2356349324 hasAuthorship W2356349324A5016207436 @default.
- W2356349324 hasAuthorship W2356349324A5031023052 @default.
- W2356349324 hasAuthorship W2356349324A5060682672 @default.
- W2356349324 hasConcept C104317684 @default.
- W2356349324 hasConcept C123894998 @default.
- W2356349324 hasConcept C147483822 @default.
- W2356349324 hasConcept C153911025 @default.
- W2356349324 hasConcept C159047783 @default.
- W2356349324 hasConcept C203014093 @default.
- W2356349324 hasConcept C22744801 @default.
- W2356349324 hasConcept C2522874641 @default.
- W2356349324 hasConcept C2777382497 @default.
- W2356349324 hasConcept C2777410769 @default.
- W2356349324 hasConcept C2779026604 @default.
- W2356349324 hasConcept C2780593183 @default.
- W2356349324 hasConcept C40767141 @default.
- W2356349324 hasConcept C54355233 @default.
- W2356349324 hasConcept C547475151 @default.
- W2356349324 hasConcept C85265743 @default.
- W2356349324 hasConcept C86803240 @default.
- W2356349324 hasConceptScore W2356349324C104317684 @default.
- W2356349324 hasConceptScore W2356349324C123894998 @default.
- W2356349324 hasConceptScore W2356349324C147483822 @default.
- W2356349324 hasConceptScore W2356349324C153911025 @default.
- W2356349324 hasConceptScore W2356349324C159047783 @default.
- W2356349324 hasConceptScore W2356349324C203014093 @default.
- W2356349324 hasConceptScore W2356349324C22744801 @default.
- W2356349324 hasConceptScore W2356349324C2522874641 @default.
- W2356349324 hasConceptScore W2356349324C2777382497 @default.
- W2356349324 hasConceptScore W2356349324C2777410769 @default.
- W2356349324 hasConceptScore W2356349324C2779026604 @default.
- W2356349324 hasConceptScore W2356349324C2780593183 @default.
- W2356349324 hasConceptScore W2356349324C40767141 @default.
- W2356349324 hasConceptScore W2356349324C54355233 @default.
- W2356349324 hasConceptScore W2356349324C547475151 @default.
- W2356349324 hasConceptScore W2356349324C85265743 @default.
- W2356349324 hasConceptScore W2356349324C86803240 @default.
- W2356349324 hasIssue "14" @default.
- W2356349324 hasLocation W23563493241 @default.
- W2356349324 hasOpenAccess W2356349324 @default.
- W2356349324 hasPrimaryLocation W23563493241 @default.
- W2356349324 hasRelatedWork W2082279628 @default.
- W2356349324 hasRelatedWork W2083207455 @default.
- W2356349324 hasRelatedWork W2172153565 @default.
- W2356349324 hasRelatedWork W2230069488 @default.
- W2356349324 hasRelatedWork W2270131876 @default.
- W2356349324 hasRelatedWork W2348772630 @default.
- W2356349324 hasRelatedWork W2349657202 @default.
- W2356349324 hasRelatedWork W2356309425 @default.
- W2356349324 hasRelatedWork W2359585664 @default.
- W2356349324 hasRelatedWork W2365451189 @default.
- W2356349324 hasRelatedWork W2380092435 @default.
- W2356349324 hasRelatedWork W2382339419 @default.
- W2356349324 hasRelatedWork W2382595882 @default.
- W2356349324 hasRelatedWork W2393864532 @default.
- W2356349324 hasRelatedWork W2399316241 @default.
- W2356349324 hasRelatedWork W2401942643 @default.
- W2356349324 hasRelatedWork W2406776306 @default.
- W2356349324 hasRelatedWork W2424176434 @default.
- W2356349324 hasRelatedWork W2443360794 @default.
- W2356349324 hasRelatedWork W3198367594 @default.
- W2356349324 hasVolume "86" @default.
- W2356349324 isParatext "false" @default.
- W2356349324 isRetracted "false" @default.
- W2356349324 magId "2356349324" @default.
- W2356349324 workType "article" @default.