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- W2356377579 abstract "【Objective】To investigate the pathogenicity of matrix metalloproteinase 3,9(MMP-3,MMP-9) regulations of human periodontal ligment cells(hPDLCs) challenged by different P.gingivalis strains.【Methods】Human periodontal ligment cells were cultured using explant culture technique.P.gingivalis W83,P.gingivalis ATCC33277 and P.gingivalis FDC381 were assessed for their inductions of MMP-3,MMP-9 expression in human periodontal ligment cells.MMP-3,MMP-9 protein levels in culture supernatant were determined by ELISA at the different time intervals(5 min,30 min,60 min,120 min,24 h,48 h) following continuous co-culture of bacteria with hPDLCs.【Results】MMP-3 and MMP-9 protein levels produced by hPDLCs co-culture with P.gingivalis W83,P.gingivalis ATCC33277 and P.gingivalis FDC381 were significantly stronger than unsimulated group(P 0.01).The concentrations of MMP-3 and MMP-9 produced by hPDLCs co-culture with the P.gingivalis W83 were more than P.gingivalis ATCC33277 and P.gingivalis FDC381(P 0.01).【Conclusion】P.gingivalis W83 has stronger pathogenicity for periodontal disease,which probably depending on the bacteria-cell interaction producing much matrix metalloproteinase in local." @default.
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- W2356377579 date "2012-01-01" @default.
- W2356377579 modified "2023-09-28" @default.
- W2356377579 title "Matrix metalloproteinase 3 and 9 regulations of human periodontal ligment cells stimulated by Porphyromonas gingivalis with different strains" @default.
- W2356377579 hasPublicationYear "2012" @default.
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