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- W2356411681 abstract "To analyze the relation between the results of ELISA and WB to test positive HIV antibody of screening test in blood donors, evaluate the need for and feasibility of WB in identifying HIV antibody, and find some existing problems. HIV antibody of 326568 blood donors was tested twice, during which all positive samples were subjects of this experiment. These samples were simultaneously tested, by comparatively accepted ELISA reagent and WB, and the relation between testing results of the two methods was compared. Samples of the same testing result account for 71.7%; the rate of positive accordance was 48.78% and the rate of negative accordance was 83.13%; With WB as a true criteria, the sensitivity of ELISA reagent of HIV antibody was 100% and specificity was 84.15%. When the positive group was compared with negative one, t=15.21,P0.001, which indicates remarkable statistical difference in S/CO between positive group and negative one. Comparing positive group with unidentified that, t=13.07,P0.001, which indicates remarkable statistical difference in S/CO between positive group and unidentified one. Comparing unidentified group with false-positive group that is negative by WB but positive by ELISA, t=2.13, P0.05, which indicates no statistical difference in S/CO between two groups and unidentified result possible is false-positive. All value of S/CO in 20 identified positive samples were greater than 5; samples whose value of S/CO were below less 1 were 71, and those between 1 and 2.999 were 19. Blood donors of unidentified HIV antibody were revisited after 6 months, whose testing results remain invariable, so they were diagnosed as negativity. The high value of S/CO(5) indicates more possibility of positive HIV antibody, but when the value is less 2.999, the possibility of positive HIV antibody is small. It is comparatively accurate for WB to identify positive and negative samples. The flaw of WB consists in unidentified results, which bring about many troubles at work." @default.
- W2356411681 created "2016-06-24" @default.
- W2356411681 creator A5035770168 @default.
- W2356411681 date "2008-01-01" @default.
- W2356411681 modified "2023-09-25" @default.
- W2356411681 title "Identification and Flaws of HIV Antibody Western Blot to Test Blood Donors" @default.
- W2356411681 hasPublicationYear "2008" @default.
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