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- W2356415642 abstract "The tender leaves of Ophioglossum thermale Kom.were used as explants,all suitable media of culture in vitro and germplasm conservation in vitro were screened through uniform design experiments.The results showed that WPM+ZT0.50 mg·L-1+2,4-D1.90 mg·L-1 was most suitable for callus induction with a induction rate of 96.5%;WPM+ZT1.70 mg·L-1 +NAA0.10 mg·L-1 was most suitable for shoots regeneration with a regeneration rate of 99.0%;1/4MS+IAA0.05 mg·L-1 +IBA0.03 mg·L-1 was most suitable for rooting with a rooting rate of 97.8%;WPM+KT1.10 mg·L-1+phloridzin3.20 mg·L-1was most suitable for in vitro germplasm preservation for 24 months with a growing rate of 6.5%.The results prove that In vitro culture system of Ophioglossum thermale has been successfully established,and the method of defering growth was practical for germplasm conservation in vitro at normal temperature." @default.
- W2356415642 created "2016-06-24" @default.
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- W2356415642 date "2011-01-01" @default.
- W2356415642 modified "2023-09-24" @default.
- W2356415642 title "Screening of media for in vitro culture and germplasm conservation in vitro of Ophioglossum thermale Kom" @default.
- W2356415642 hasPublicationYear "2011" @default.
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