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- W2356501162 abstract "Objective To investigate the frequency distribution of apoliporotein E(apo E) gene polymorphism in the KarzaKan natural population in Shihezi area in Xinjiang and establish the KarzaKan nationality apo E genetic resource reservoir.To establish a rapid and sensitive method for apo E genotyping. Methods The apo E gene sequences containing amino acid positions 112 and 158 were amplified by polymerase chain reaction(PCR).The PCR products were digested with Hha I and subjected to electrophoresis on 12% polyacrylamide gels.The patterns of restriction fragment length polymorphism(RFLP) of apo E gene were distinguished. Results The prevalence of apo E genotype E3/3,E3/4,E2/3,E2/4,E4/4 in KarzaKan population in Shihezi area in Xinjiang were 65%,17%,16%,1% and 1% respectively.The apo E allelic frequencies were 9.0% for e2,81.5% for e3,9.5% for e4.The frequence of e2 was higher than that of the Uighur nationality and e4 was significantly lower than that of the Uighur nationality,but there was no statistical significance. Conclusions The PCR-RFLP method is a rapid and accurate technique.This method is suitable for routine laboratories and large-scale population studies." @default.
- W2356501162 created "2016-06-24" @default.
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- W2356501162 date "2007-01-01" @default.
- W2356501162 modified "2023-09-25" @default.
- W2356501162 title "Determination of apolipoprotein E gene polymorphism in the KarzaKan population in Shihezi area in Xinjiang" @default.
- W2356501162 hasPublicationYear "2007" @default.
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