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- W2356509139 abstract "In this study,the gene of mesophilic α-amylase( amy L) from a mesophilic α-amylase-producing strain( Bacillus subtilis 264) has been cloned into an expression plasmid p AX01 at Bam HI and Sac II sites,forming an integrative recombinant plasmid p AX01- amy L. Following its transformation into B. subtilis 264,the recombinant plasmid p AX01- amy L mediates chromosomal integration of the amy L expression cassette by homologous recombination at lac A locus,and therefore,increases the copy number of the amy L genein B. subtilis 264. As a proof of concept,a high-level expression transformant,designated as B. subtilis ZHWY,was isolated and verified by Southern blot. It was shown that the secreted α-amylase activity of B. subtilis ZHWY in a 500 m L shake flask reached 730 U /m L( 156 U / mg protein) which was an improvement of 70% when compared with the wildtype strain B. subtilis264. Moreover,in a 5 L fermenter,B. subtilis ZHWY afforded a high α-amylase activity of 1450 U / m L,demonstrating its good potential for the industrial production of mesophilic α-amylase." @default.
- W2356509139 created "2016-06-24" @default.
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- W2356509139 date "2015-01-01" @default.
- W2356509139 modified "2023-09-25" @default.
- W2356509139 title "High-level expression of mesophilic α-amylase in Bacillus subtilis by chromosomal integration" @default.
- W2356509139 hasPublicationYear "2015" @default.
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