Matches in SemOpenAlex for { <https://semopenalex.org/work/W2356522946> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2356522946 abstract "Objective To explore the effect of specific small interference RNA of hypoxia induc- ible factor-1 a on enhancing sensitivity of hcpatocellular carcinoma to chemotherapeutic drags.Methods PSilencer2.1/HIF-1α-siRHA eukaryotic expression plasmid was constructed.Liposome carrying the plas- mid was stably transfected into C28 hepatocarcinoma cell line resistant to multiple chemotherapeutic drugs. The whole experiments were divided into three groups:experiment group,negative control group and empty control group.Real-time fluorescent quantitative PCR and Western-blot technique were respectively used to analyze the expression of MDR1,MRP1 and LRP at mRNA and protein level in the above three group cells.Flow eytometry was used to determine apoptosis index of those cells after being administrated by 5- Fu.Randomly,every group of cells was subcutaneously inoculated into 10 nude mice.After subcutaneous neoplasm having grown,their sensitivities to ADM were compared among these three groups.Results HIF-1α-siRNA could specifically inhibit HIF-1α expression.Moreover,HIF-1α-siRNA made the expres- sion amounts of MDR1,MRP1,LRP at mRNA and protein level decrease in C28 hepatocarcinoma cell line.At 24h,48h and 72h after administration of 5-Fu,apoptosis index of the experiment group was respec- tively 2.88,3.56 and 3.87 folds of that of negative control group,which suggested the sensitivity of experi- ment group to 5-Fu was significantly increased.Average size of subcutaneous neoplasm of nude mice in ex- periment group was remarkably reduced after being treated by ADM and tumor inhibiting rate was 41.35%,which was significantly different from negative control group(P0.01).Conclusion HIF-1α- siRNA expressed plasmid is successfully constructed.SiRNA targeted HIF-1α gene could enhance the sen- sitivity of hepatocareinoma cells to chemotherapeutic drugs.And an effective therapeutic strategy for hepa- tocellular carcinoma could possibly be realized by united administration of conventional chemotherapeutic agents and specific siRNA of HIF-1α." @default.
- W2356522946 created "2016-06-24" @default.
- W2356522946 creator A5032136357 @default.
- W2356522946 date "2007-01-01" @default.
- W2356522946 modified "2023-09-23" @default.
- W2356522946 title "Enhancing sensitivity of Hepatocarcinoma cells to chemotherapeutic drugs by small interference RNA of hypoxia inducible factor-1α" @default.
- W2356522946 hasPublicationYear "2007" @default.
- W2356522946 type Work @default.
- W2356522946 sameAs 2356522946 @default.
- W2356522946 citedByCount "0" @default.
- W2356522946 crossrefType "journal-article" @default.
- W2356522946 hasAuthorship W2356522946A5032136357 @default.
- W2356522946 hasConcept C104317684 @default.
- W2356522946 hasConcept C105580179 @default.
- W2356522946 hasConcept C1491633281 @default.
- W2356522946 hasConcept C153911025 @default.
- W2356522946 hasConcept C166703698 @default.
- W2356522946 hasConcept C178790620 @default.
- W2356522946 hasConcept C185592680 @default.
- W2356522946 hasConcept C190283241 @default.
- W2356522946 hasConcept C22615655 @default.
- W2356522946 hasConcept C2776415932 @default.
- W2356522946 hasConcept C502942594 @default.
- W2356522946 hasConcept C540031477 @default.
- W2356522946 hasConcept C54009773 @default.
- W2356522946 hasConcept C54355233 @default.
- W2356522946 hasConcept C553184892 @default.
- W2356522946 hasConcept C55493867 @default.
- W2356522946 hasConcept C67705224 @default.
- W2356522946 hasConcept C7836513 @default.
- W2356522946 hasConcept C81885089 @default.
- W2356522946 hasConcept C86803240 @default.
- W2356522946 hasConceptScore W2356522946C104317684 @default.
- W2356522946 hasConceptScore W2356522946C105580179 @default.
- W2356522946 hasConceptScore W2356522946C1491633281 @default.
- W2356522946 hasConceptScore W2356522946C153911025 @default.
- W2356522946 hasConceptScore W2356522946C166703698 @default.
- W2356522946 hasConceptScore W2356522946C178790620 @default.
- W2356522946 hasConceptScore W2356522946C185592680 @default.
- W2356522946 hasConceptScore W2356522946C190283241 @default.
- W2356522946 hasConceptScore W2356522946C22615655 @default.
- W2356522946 hasConceptScore W2356522946C2776415932 @default.
- W2356522946 hasConceptScore W2356522946C502942594 @default.
- W2356522946 hasConceptScore W2356522946C540031477 @default.
- W2356522946 hasConceptScore W2356522946C54009773 @default.
- W2356522946 hasConceptScore W2356522946C54355233 @default.
- W2356522946 hasConceptScore W2356522946C553184892 @default.
- W2356522946 hasConceptScore W2356522946C55493867 @default.
- W2356522946 hasConceptScore W2356522946C67705224 @default.
- W2356522946 hasConceptScore W2356522946C7836513 @default.
- W2356522946 hasConceptScore W2356522946C81885089 @default.
- W2356522946 hasConceptScore W2356522946C86803240 @default.
- W2356522946 hasLocation W23565229461 @default.
- W2356522946 hasOpenAccess W2356522946 @default.
- W2356522946 hasPrimaryLocation W23565229461 @default.
- W2356522946 hasRelatedWork W157584257 @default.
- W2356522946 hasRelatedWork W2000943930 @default.
- W2356522946 hasRelatedWork W2171021566 @default.
- W2356522946 hasRelatedWork W2182481622 @default.
- W2356522946 hasRelatedWork W2395554262 @default.
- W2356522946 hasRelatedWork W2406772038 @default.
- W2356522946 hasRelatedWork W2407477249 @default.
- W2356522946 hasRelatedWork W2419151126 @default.
- W2356522946 hasRelatedWork W2419534933 @default.
- W2356522946 hasRelatedWork W2439085213 @default.
- W2356522946 hasRelatedWork W2465633368 @default.
- W2356522946 hasRelatedWork W2467530266 @default.
- W2356522946 hasRelatedWork W2885098366 @default.
- W2356522946 hasRelatedWork W2978707576 @default.
- W2356522946 hasRelatedWork W3030246978 @default.
- W2356522946 hasRelatedWork W3030501259 @default.
- W2356522946 hasRelatedWork W3031206425 @default.
- W2356522946 hasRelatedWork W3031755134 @default.
- W2356522946 hasRelatedWork W3032143052 @default.
- W2356522946 hasRelatedWork W3032595020 @default.
- W2356522946 isParatext "false" @default.
- W2356522946 isRetracted "false" @default.
- W2356522946 magId "2356522946" @default.
- W2356522946 workType "article" @default.