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- W2356523502 abstract "Acrossocheilus monticola is the endemic fish in the upper reach of the Yangtze River.For a long time,little attention has been paid to this population because of its small size and low economic value.In recent years,the construction of hydroelectric projects and some anthropic factors changed the physical environment and hydrological conditions in the Yangtze River,which lead to some impacts on the rare and endemic fishes in the Yangtze River including A.monticola.In this study,we isolated microsatellite loci from A.monticola genome by FIASCO(Fast Isolation by AFLP Sequences Containing Repeats) procedure.Taking advantages of biotinylated oligo probes(AC)8,(CT)8,(GGT)8,(GATA)8 and(GATT)7,we successfully constructed a microsatellite-enriched genomic library,and 495 clones were sequenced.163 pairs of SSR-primers were designed afterwards,and from which 18 polymorphic microsatellite markers were screened out by PCR amplification.Using these polymorphic microsatellite markers,we analyzed the genetic diversity of the population of A.monticola collected from the Chishui city.The results showed that the average allele number of microsatellite loci was 19.6,the average observed heterozygosity(Ho) was 0.8312,and the average expected heterzygosity(He) was 0.8701.Six markers,Am07,Am35,Am47,Am69,Am78 and Am127,were deviated from Hardy-Weinberg equilibrium significantly(P0.05).These results indicated that the genetic diversity of A.monticola was diversiform,and these polymorphic microsatellite primers could be used in A.monticola population genetic analysis as well as biological resource conservation." @default.
- W2356523502 created "2016-06-24" @default.
- W2356523502 creator A5041866484 @default.
- W2356523502 date "2013-01-01" @default.
- W2356523502 modified "2023-09-23" @default.
- W2356523502 title "Isolation and Characterization Analysis of Microsatellite Loci in Acrossocheilus monticola" @default.
- W2356523502 hasPublicationYear "2013" @default.
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