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- W2356524253 abstract "Objective:To construct a recombinant adenovirus Ad-MOMP carrying the major outer membrane protein(MOMP) gene of Chlamydia trachomatis serovar E,and provide the basis for further research on Chlamydia trachomatis adenovirus vaccine.Methods: The MOMP gene fragments of Chlamydia trachomatis serovar E were amplified from genome DNA by PCR method.They were cloned into pcDNAII vector and then were subcloneded into the pshuttle vector pDC316.The pshuttle vector pDC316-MOMP and the genomic plasmids PBHGlox△E1,3Cre were co-transfected into 293 cells for package and amplification of recombinant adenovirus Ad-MOMP,which was identified by PCR and RT-PCR.The BALB/c mice immunized with recombinant adenovirus and anti-MOMP specific IgG in serum were tested.Results: 1.1?kb gene fragment was amplified from genome DNA by PCR method as being anticipated.Recombinant pshuttle vectors,pDC316-MOMP were confirmed by enzyme digestion and nucleotide sequencing.Recombinant adenovirus Ad-MOMP was verified byPCR and RT-PCR.AntiMOMP specific IgG antibodies were induced in mice by recombinant adenovirus AdMOMP.Conclusion: The recombinant adenovirus Ad-MOMP has been successfully constructed,and it is able to induce Anti-MOMP specific antibodies in mice." @default.
- W2356524253 created "2016-06-24" @default.
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- W2356524253 date "2006-01-01" @default.
- W2356524253 modified "2023-09-25" @default.
- W2356524253 title "Construction and immunogenicity of recombinant adenovirus carrying the major outer membrane protein gene of Chlamydia trachomatis seovar E" @default.
- W2356524253 hasPublicationYear "2006" @default.
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