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- W2356534564 abstract "Objective To establish a simple and rapid molecular typing method for vibrio cholerae based on randomly amplified polymorphic DNA(RAPD) analysis.Methods A total of 16 representative strains of V.cholerae,including members of the O_1,O_(139),and non-O_1/non O_(139) serogroups,and a total of 40 random primers were used to develop the RAPD-PCR experiments,in order to screen out the ideal primers by which the obtained DNA fingerprinting patterns must have high polymorphism and sufficient resolving power in molecular typing.Results After a series of RAPD-PCR experiments combined statistical clustering analysis,two most satisfactory primers were selected from the 40 random primers.A finer resolution of the clustering of the toxigenic O_1/O_(139),non-toxigenic O_1 and non-O_1/non O_(139) subtypes were obtained by using these two primers in RAPD-PCR for the 16 strains of V.cholerae.Conclusion In this study,we screened out two ideal random primers and established the corresponding RAPD-based molecular typing method.This method is easy,timesaving and labor-saving,especially suitable for the basic laboratories of CDC and port quarantine departments to perform surveillance and epidemiological traceability of cholera." @default.
- W2356534564 created "2016-06-24" @default.
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- W2356534564 date "2012-01-01" @default.
- W2356534564 modified "2023-09-27" @default.
- W2356534564 title "Establishment of molecular typing method for vibrio cholerae based on randomly amplified polymorphic DNA analysis" @default.
- W2356534564 hasPublicationYear "2012" @default.
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