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- W2356536638 abstract "Aim: To explore the differences in gene profiling between cardia and esophageal adenocarcinoma by cDNA and tissue array.Methods:Tissue specimens of 10 cases of CAC,9 cases of EAC,7 cases of Barrett's esophagus(BE) and 6 cases of cardia intestinal metaplasia(CIM) were collected.cDNA microarray was used to screen the gene expression levels between CAC and EAC,CIM and BE,respectively,while immunohistochemical staining of the different formalin fixed paraffin-embedded tumor specimens was used to detect the proteins.Results: The differentially expressed genes between CAC and EAC were compared with those between CIM and BE,and 23 genes expressed in differential trends,of which 17 were up-regulated and 6 were down-regulated.Some differentially expressed genes including BCRP,cIAP1 and BCRP were validated by immunohistochemical staining of formalin fixed paraffin-embedded tumor specimens.The positive rates of BCRP,cIAPT were 80.0% in CAC,33.3% in EAC,and both of the positive rates in CAC were higher than those in EAC(P=0.018).The positive rate of MMP2 in CAC was 40.0%,which had no difference from 55.5% in EAC(P=0.286).Conclusion: The characteristics of gene expression in mRNA and protein level between CAC and EAC were different significantly,which indicated that CAC and EAC might not be the same origin." @default.
- W2356536638 created "2016-06-24" @default.
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- W2356536638 date "2008-01-01" @default.
- W2356536638 modified "2023-09-23" @default.
- W2356536638 title "Comparison of gene profiling between cardia and esophageal adenocarcinoma" @default.
- W2356536638 hasPublicationYear "2008" @default.
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