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- W2356556904 abstract "Litopenaeus vannamei,native to the Western Pacific Coast of Latin America,was introduced to China in 1988,and now contributes over 80% of China’s shrimp production.However,cold wave weather during recent winters of southern China led heavy losses to the culture industry of Litopenaeus vannamei.So,breeding of cold-tolerant variety is an urgent task,and molecular marker assisted selecting is an effective method for selective breeding.To study the mo-lecular mechanism of cold tolerance,we cloned TCP-1-Beta homolog gene of Litopenaeus vannamei,and studied its relationship with cold tolerance.Based on the sequence from electronic cloning,a pair of primers were designed,and a 1691 bp cDNA was obtained by RT-PCR.The cDNA contained 1608 bp ORF which encoding a peptide of 535aa.Tissue expression pattern by real-time PCR revealed that TCP-1-Beta mRNA mainly existed in hepatopancreas tissue.Cold induction by different temperatures revealed that TCP-1-Beta mRNA expression started to increase after 13℃ treatment for 24 hours,and the increase was remarkable after 11℃ treatment.As for 13℃ treatment for different times,the ex-pression pattern showed no changes during the first 24 hours,but significantly increased after 36 hours treatment.A SNP(single nucleotide polymorphism) site was found in 420 bp of ORF,and Genetype of 216 Litopenaeus vannamei were determined by PCR-RFLP.Variance analysis revealed that the SNP Genotype was significantly related with cold toler-ance parameter CDH(cooling degree hours),and CDH for GG type Litopenaeus vannamei was longer than AA type,which provided a potential marker for selective breeding of cold tolerance variety." @default.
- W2356556904 created "2016-06-24" @default.
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- W2356556904 date "2011-01-01" @default.
- W2356556904 modified "2023-09-25" @default.
- W2356556904 title "MOLECULAR CLONING OF LITOPENAEUS VANNAMEI TCP-1-BETA GENE AND ANALYSIS ON ITS RELATIONSHIP WITH COLD TOLERANCE" @default.
- W2356556904 hasPublicationYear "2011" @default.
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