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- W2356559550 abstract "Based on the sequence of WNV E domainⅢ gene in GenBank,a pair of primers was designed to amplify domainⅢ gene with PCR.Then was cloned into Drosophila Expression System to get recombinant expressed vector(pMT-DⅢ).After co-transfection of the pMT-DⅢ and pCoBlast into S2 cells.The positive S2 cells were further screened with 12 mg/L Blasticidin.pMT-Ⅲ was transfected into S2 cells which was named S2-DⅢ.S2-DⅢ was induced by copper sulfate.S2-DⅢ cell which could permanently express recombinant DⅢ protein.The assessment of purity and specificity of WNV DⅢ was identified by SDS-PAGE and Western blotting." @default.
- W2356559550 created "2016-06-24" @default.
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- W2356559550 date "2008-01-01" @default.
- W2356559550 modified "2023-09-23" @default.
- W2356559550 title "Expression and identification of WNV E domainIII in drosophila S2 cell lines" @default.
- W2356559550 hasPublicationYear "2008" @default.
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