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- W2356570252 abstract "Alkaline protease was immobilized by using porous chitosan microspheres which were prepared by ourselves,the proportion of enzyme to carriers,the concentrations of alkaline protease,glutaraldehyde and BSA,pH,temperature,absorption and crosslinking time were studied by using recycling rate of activity as index and the better method was found.The test indicated that the optimum technics were as follows: the proportion of enzyme to carriers was 315 U/g,the concentration of alkaline protease,glutaraldehyde and BSA were 45 mg/mL,1%,4.5 mg/mL respectively,pH was 7.2,temperature was 4 ℃,absorption and crosslinking time were 48 hours and 8 hours,the recycling rate of activity reached 65.46%,the alkaline protease has good physical and chemical properties." @default.
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- W2356570252 date "2008-01-01" @default.
- W2356570252 modified "2023-09-26" @default.
- W2356570252 title "Study on immobilization of alkaline proteinase on porous chitosan microspheres" @default.
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