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- W2356579924 abstract "AIM:To investigate the effect of lysophosphatidic acid(LPA) on the expression of integrin B_6(ITGB6) for determining the role of transforming growth factor B(TGF-B) activation induced by LPA in this process. METHODS:Normal human bronchial epithelial(NHBE) cells were primarily cultured in 6 well plate and stimulated with LPA.The mRNA expression of ITGB6 and the level of cell surface ITGB6 protein were detected by RT-PCR and flow cytometry, respectively.The activity of active TGF -βinduced by LPA was measured by the method of transformed mink lung epithelial cells(TMLC) transfected with TGF -βresponsive plasminogen activator inhibitor 1(PAI-1 ) promoter fused with firefly luciferase reporter gene.RESULTS:After stimulated with LPA at concentration of 10μmol/L for 2 h,the mRNA expression of ITGB6 in epithelial cells was significantly increased in a time-dependent manner.The results of flow cytometry showed that the protein level of ITGB6 on cell surface was obviously increased after treated with LPA at concentration of 10μmol/L for 4 h.The active TGF -βinduced by LPA in epithelial cells was blocked by anα_vB_6 blocking antibody. However,α_vP_6 blocking antibody failed to inhibit the mRNA expression of ITGB6 induced by LPA.CONCLUSION: LPA induces the mRNA and cell surface protein of ITGB6 in epithelial cells.The up-regulated ITGB6 expression by LPA is independent on LPA-induced TGF -βactivation." @default.
- W2356579924 created "2016-06-24" @default.
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- W2356579924 date "2012-01-01" @default.
- W2356579924 modified "2023-09-25" @default.
- W2356579924 title "Effect of lysophosphatidic acid on expression of integrin β_6 in epithelial cells" @default.
- W2356579924 hasPublicationYear "2012" @default.
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