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- W2356608420 abstract "Objective [WT5”BZ] To study the relationship between human papillomavirus (HPV) 16 and its serum antibody in the development of gastric carcinoma (GC) [WT5”HZ] Methods [WT5”BZ] Polymerase chain reaction (PCR) technique was used to detect HPV DNA in 42 fresh GC specimens and 42 fresh normal gastric mucosa adjacent to the tumour (NGMAT). Enzyme linked immunosorbent assay (ELISA) was used to screen the serum HPV16 antibody(Ab) of 42 GC patients and 46 controls using HPV16 virus like particles (VLPs) which was produced by recombinant bacilovirus in insect cells [WT5”HZ] Results [WT5”BZ] HPV16 DNA was found in 26 2% (11/42) of GC specimens,but in none (0/42) of NGMAT ( P 0 01) There was a significant difference of serum mean HPV16Ab A value between GC patients (0 069±0 026) and the control group (0 036±0 027) ( P 0 05),and the difference was also significant between HPV16 DNA positive GC patients (0 081±0 027) and negative ones (0 042±0 016) ( P 0 01). [WT5”HZ]Conclusions [WT5”BZ] The infection of HPV16 is likely to be involved in the development of GC The assay of serum HPV16 Ab could be used as an index for the serum diagnosis of HPV16 infections and a method in immunological study of HPV16 associated tumours [WT5”HZ]" @default.
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- W2356608420 date "2000-01-01" @default.
- W2356608420 modified "2023-09-23" @default.
- W2356608420 title "Association of human papillomavirus 16 and its serum antibody in gastric carcinoma" @default.
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