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- W2356633555 abstract "Objective To investigate the effects of hairpin RNA on the expression of human papillomavirus E6 gene. Methods Plasmids expressing hairpin RNA, sense RNA and anti-sense RNA were constructed. The recombinants were transfected into cervical cancer cell line, CaSKi. Expression of E6 was detected with semi-quantitative RT-PCR and Western blot. Results Hairpin RNA could significantly reduce the expressions of E6 mRNA and protein, which was more efficient than traditional anti-sense RNA did. Conclusion The RNA interference through hairpin RNA can effectively inhibit the expression of target gene, and can be potentially useful in gene therapy of HPV related tumors." @default.
- W2356633555 created "2016-06-24" @default.
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- W2356633555 date "2004-01-01" @default.
- W2356633555 modified "2023-09-28" @default.
- W2356633555 title "The Effects of Hairpin RNA on the Expression of Human Papillomavirus E6 Gene" @default.
- W2356633555 hasPublicationYear "2004" @default.
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