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- W2356665219 abstract "Aim To identify ehrlichial pathogens in Boophilus microplus Methods A nested PCR specific for tick transmitted Ehrlichia sp was established with 2 pairs of primers designed based on the 16S rDNA sequences of Ehrlichia The DNA samples of B microplus(each sample was prepared from 2 ticks) were detected by the nested PCR The 3′ end fragments of 16S rDNA of the positive samples in nested PCR were cloned and the cloned fragments(～450bp) were sequenced Results Sixteen of 43 DNA samples(37%) of the ticks from Xizang were positive in nested PCR,but the samples from Sichuan were all negative Two different 16S rDNAs were identified in the positive samples,one identical to that of Anplasma marginale and other closely related to that of E chaffeensis with a difference of 7 bases(～1 6%) Conclusion E chaffeensis-like organism and A Marginale are carried by B microplus in Xizang;the E chaffeensis-like organism may be a new species of the genus Ehrlichia" @default.
- W2356665219 created "2016-06-24" @default.
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- W2356665219 date "2002-01-01" @default.
- W2356665219 modified "2023-09-24" @default.
- W2356665219 title "Detection of 16S rDNA of Ehrlichia chaffeensis-like organism and Anplasma marginale in Boophilus microplus collected from Xizang" @default.
- W2356665219 hasPublicationYear "2002" @default.
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