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- W2356673579 abstract "Objective To study the expression,identification and enzyme kinetics of pCold II-His-PRL-3 expression vector.Methods pCold II-His-PRL-3 expression vector was constructed and then transfected into ArcticExpress E.coli.Expression of His-PRL-3 fusion protein was induced by IPTG.After purified by Ni2+-NTA resin,the purified protein was verified with SDS-PAGE,Western blot,MALDI-TOF-TOF and enzyme kinetics analysis.Results The pCold II-His-PRL-3 expression vector included right coding sequence of PRL-3.The fusion protein was expressed in ArcticExpress E.coli after IPTG induction,and had antigenicity using Western blot.Mass chromatographic analysis showed that the amino acid sequence of target protein was consistent with that of PRL-3 protein.Michaelis constant(Km) and catalytic constant(Kcat) of PRL-3 enzyme were 6.59 μmol/L and 0.44 min-1,respectively.Conclusion The constructed pCold II-His-PRL-3 expression vector can express PRL-3 protein with enzyme activity,which provides the foundation for screening PRL-3 enzyme inhibitor." @default.
- W2356673579 created "2016-06-24" @default.
- W2356673579 creator A5077549494 @default.
- W2356673579 date "2011-01-01" @default.
- W2356673579 modified "2023-09-27" @default.
- W2356673579 title "Expression,identification and enzyme kinetics analysis of recombinant human PRL-3 protein" @default.
- W2356673579 hasPublicationYear "2011" @default.
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