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- W2356700826 abstract "AIM:To construct HBx eukaryotic expression vector pEYFP-C1-X and eukaryotic expression vector pGL3-P4 driven by P4 promoter of human IGF-II gene and to investigate the effect of HBx on the transcription activity of IGF-II gene P4 promoter. METHODS:HBx gene and P4 promoters were cloned into pEYFP-C1 and pGL3-basic vectors respectively by gene recombination techniques to construct recombinant plasmids pEYFP-C1-X and pGL3-P4. HepG2 cells were transfected with pEYFP-C1-X and the resistant cell clones were selected by G418. Then methylated pGL3-P4 was transiently transfected into the above cell clones,and the transcription activity of P4 promoter was determined by dual-luciferase reporter assay system. RESULTS:(1) Aim fragments HBx gene and P4 promoter that were cloned were 465 bp and 1 246 bp,respectively and the DNA sequences were accordant with GenBank data confirmed by restricted enzyme digestion and sequencing. (2) HepG2-EYFP-X cells that expressed HBx protein were obtained. (3) Luciferase activity of methylated P4 promoter in the HepG2-EYFP-X was more than that of control cell HepG2-EYFP (P0.01). CONCLUSION:HBx may enhance the transcription activity of the P4 promoter." @default.
- W2356700826 created "2016-06-24" @default.
- W2356700826 creator A5061494199 @default.
- W2356700826 date "2010-01-01" @default.
- W2356700826 modified "2023-09-24" @default.
- W2356700826 title "Effect of HBx on transcriptional activity of IGF-II gene P4 promoter" @default.
- W2356700826 hasPublicationYear "2010" @default.
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