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- W2356720018 abstract "Objective:To knock_out Shigella flexneri asd gene quickly with Red system.Methods:A pair of primers, which are homologous to asd gene and chloramphenicol_resistant gene, were synthesized. The chloramphenicol_resistant gene was then amplified by PCR with the primers. The PCR products were electro_transferred into Escherichia coli strain DH5α and S.flexneri 2a strain 2457T. With the help of Red recombinant system, asd genes of DH5α and 2457T were in vivo replaced by chloramphenicol gene. Due to FRT sites flanking chloramphenicol gene, the latter was eliminated by FLP_promoted recombination events. Results and Conclusions:The mutants of E.coli and S.flexneri ,from which asd gene was completely deleted, were constructed and were chloramphenicol sensitive. This work indicates that Red system can be successfully applied to other bacteria besides E.coli ." @default.
- W2356720018 created "2016-06-24" @default.
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- W2356720018 date "2002-01-01" @default.
- W2356720018 modified "2023-09-24" @default.
- W2356720018 title "Quick knockout of Shigella flexneri asd gene with Red system" @default.
- W2356720018 hasPublicationYear "2002" @default.
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