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- W2356775314 abstract "In this study,the clinical and pathological symptoms of 6 cloned transgenic Large White dying of transmissible gastroenteritis were recorded,and the organ coefficients of multiple tissues between these transgenic pigs and normal Large White pigs were compared. Conventional PCR and Long PCR were performed to amplify the exogenous p EF-BMPRIB( CDS)-Neo vector,and quantitative real-time PCR( q RT-PCR) was used to detect the copy numbers of exogenous NEO by absolute quantification assay in these transgenic pigs. The results showed that all of the sick cloned transgenic piglets exhibited diarrhea and stool abnormality,and some had fever and cough. The dissection revealed that all these pigs had intestine swelling and milk curd in stomach,and some piglets showed congestion in small intestine. Organ coefficient analyses indicated that no significant difference was found in the detected tissues( P0. 05) except the kidney( P0. 05) between transgenic and non-transgenic pigs. Molecular identification revealed that 6 cloned transgenic pigs were transgenic positive individuals,whereas with loss or disruption of exogenous vector at different positions. Of the 6 transgenic pigs,4 lost or had disruptions in the promoter or CDS regions. The copy numbers of exogenous NEO in the 6 transgenic pigs were 1,2,2,3,4 and 5. In conclusion,cloned transgenic Large White piglets dying of transmissible gastroenteritis showed no other abnormalities except the typical clinic and pathological anatomy symptoms of this disease. Only slight difference was found in the organ coefficients between cloned transgenic and normal Large White piglets. There was no evidence that transgenic or cloning techniques led to significant changes in recipient piglets. Molecular identification indicates that these cloned pigs are transgenic positive individuals,but the exogenous gene did not completely integrate into the host genome." @default.
- W2356775314 created "2016-06-24" @default.
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- W2356775314 date "2015-01-01" @default.
- W2356775314 modified "2023-09-28" @default.
- W2356775314 title "Clinic,Pathological Anatomy Symptoms and Preliminary Molecular Identification of Cloned Transgenic Pigs Dying of Transmissible Gastroenteritis" @default.
- W2356775314 hasPublicationYear "2015" @default.
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