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- W2356780287 abstract "Objective To improve some former culture methods of primary rat cortical neurons and get pure and long living cells for in vitro study.Methods Trypsin digestion and mechanical dissociation were adopted to conduct the culture.Morphological changes of neuron cells were observed under an inversion phase contrast microscope.Immunostaining of microtubule associated protein 2(MAP2)was applied to assess cell purity of the culture.Results Viability of the conducted cells was higher than before.Cells grew well under the given condition.Most cells had one or two ecptoms grown and their interlacing looked like a net on the fourth day after planting.After the neurons grew successively for 8 d,the cell body became bigger and the prominences were thicker and there were more branches than that at the initial stage.The prominences of neurons interlaced very well even to form a typical nerve fiber net.On the tenth day,purity of the cultured neurons was over 90% by immunohistochemical identification.Conclusions Method introduced in the paper is a simple technique that can be used for in vitro primary culture of rat cortical neurons." @default.
- W2356780287 created "2016-06-24" @default.
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- W2356780287 date "2013-01-01" @default.
- W2356780287 modified "2023-09-24" @default.
- W2356780287 title "B27 primary culture of neonatal rat cortical neurons and MAP2 identification" @default.
- W2356780287 hasPublicationYear "2013" @default.
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