Matches in SemOpenAlex for { <https://semopenalex.org/work/W2356815511> ?p ?o ?g. }
Showing items 1 to 62 of
62
with 100 items per page.
- W2356815511 abstract "Five isolates of encephalomyocarditis virus were isolated from clinical tissue samples(originated) from suspected cases of intensive pig farms.Two isolates of them(BJC3 and HB1) were systematically identified.The results showed that round virus particles with diameter of 27 nm were observed by immune electron microscopy.The physicochemical character tests indicated that the isolates BJC3 and HB1 were not able to endure acid and insensitive to chloroform.However,the two isolates had different sensitivity to trypsin treatment.Exposed under 60 ℃ for 1 h,the viruses could be inactivated and protection of bivalent cation at temperature of 50 ℃ was negligible.The specific fluorescence was observed in the cytoplasm of BHK-21 cells infected with the isolates by indirect fluorescence assay using polyclonal antibody against EMCV.The VP1 gene and partial segment of 3D gene of the two isolates were amplified by RT-PCR and sequenced.Sequence analysis revealed that the nucleotide identity of VP1 gene was 99.49% and the predicted amino acid identity was 98.46% among the two isolates.The nucleotide identity of VP1 genes between EMCV strains in GenBank database and the isolates ranged from 81.61% to 99.59%.Correspondingly,the amino acid identity was above 95.5%.The identity of 3D gene amplified of both the nucleotide and amino acid was 100% among the two isolates and other EMCV strains.Phylogenetic analysis based on the nucleotide sequences of VP1 gene of EMCV showed that the two isolates were located on the backbone of the phylogenetic tree,with lesser variation.These results suggest that EMCV infection exist in pig farms in China." @default.
- W2356815511 created "2016-06-24" @default.
- W2356815511 creator A5029354745 @default.
- W2356815511 date "2007-01-01" @default.
- W2356815511 modified "2023-09-23" @default.
- W2356815511 title "Isolation and Characterization of Porcine Encephalomyocarditis Virus" @default.
- W2356815511 hasPublicationYear "2007" @default.
- W2356815511 type Work @default.
- W2356815511 sameAs 2356815511 @default.
- W2356815511 citedByCount "2" @default.
- W2356815511 countsByYear W23568155112016 @default.
- W2356815511 crossrefType "journal-article" @default.
- W2356815511 hasAuthorship W2356815511A5029354745 @default.
- W2356815511 hasConcept C104317684 @default.
- W2356815511 hasConcept C153911025 @default.
- W2356815511 hasConcept C159047783 @default.
- W2356815511 hasConcept C159654299 @default.
- W2356815511 hasConcept C193252679 @default.
- W2356815511 hasConcept C2522874641 @default.
- W2356815511 hasConcept C515207424 @default.
- W2356815511 hasConcept C54355233 @default.
- W2356815511 hasConcept C79029880 @default.
- W2356815511 hasConcept C84148353 @default.
- W2356815511 hasConcept C86803240 @default.
- W2356815511 hasConcept C956191 @default.
- W2356815511 hasConceptScore W2356815511C104317684 @default.
- W2356815511 hasConceptScore W2356815511C153911025 @default.
- W2356815511 hasConceptScore W2356815511C159047783 @default.
- W2356815511 hasConceptScore W2356815511C159654299 @default.
- W2356815511 hasConceptScore W2356815511C193252679 @default.
- W2356815511 hasConceptScore W2356815511C2522874641 @default.
- W2356815511 hasConceptScore W2356815511C515207424 @default.
- W2356815511 hasConceptScore W2356815511C54355233 @default.
- W2356815511 hasConceptScore W2356815511C79029880 @default.
- W2356815511 hasConceptScore W2356815511C84148353 @default.
- W2356815511 hasConceptScore W2356815511C86803240 @default.
- W2356815511 hasConceptScore W2356815511C956191 @default.
- W2356815511 hasOpenAccess W2356815511 @default.
- W2356815511 hasRelatedWork W123509773 @default.
- W2356815511 hasRelatedWork W2009111813 @default.
- W2356815511 hasRelatedWork W2087557066 @default.
- W2356815511 hasRelatedWork W2108115508 @default.
- W2356815511 hasRelatedWork W2132855884 @default.
- W2356815511 hasRelatedWork W2154422522 @default.
- W2356815511 hasRelatedWork W2231416552 @default.
- W2356815511 hasRelatedWork W2351354402 @default.
- W2356815511 hasRelatedWork W2352233200 @default.
- W2356815511 hasRelatedWork W2361782854 @default.
- W2356815511 hasRelatedWork W2372279304 @default.
- W2356815511 hasRelatedWork W2384457393 @default.
- W2356815511 hasRelatedWork W2417903694 @default.
- W2356815511 hasRelatedWork W2418185472 @default.
- W2356815511 hasRelatedWork W2758896821 @default.
- W2356815511 hasRelatedWork W2790179463 @default.
- W2356815511 hasRelatedWork W3139687598 @default.
- W2356815511 hasRelatedWork W612250373 @default.
- W2356815511 hasRelatedWork W636542614 @default.
- W2356815511 hasRelatedWork W2612081497 @default.
- W2356815511 isParatext "false" @default.
- W2356815511 isRetracted "false" @default.
- W2356815511 magId "2356815511" @default.
- W2356815511 workType "article" @default.