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- W2356856787 abstract "Ahighperformance liquid chromatograph(HPLC)withanelectrochemical detector(ECD)and a NUˉCLEOSIL R C 18 column(250mm×4mm,i.d.10μm)was used for the determination of histamine(HA)and5-hydroxytryptamine(5-HT)in rat′s hypothalamus.The mobile phase was a mixture of NaH 2 PO 4 (25mmol/L,containing0.5mmol/LdisodiumEDTAand3mmol/Lsodiuml-octanesulfonate at pH4.8)and acetonitrile in a ratio of100∶14(by volume).The flowrate was0.8mL/min,and the detector voltage was set at0.6V.The homogenate of rat′s hypothalamus was centrifuged at4℃.A part of the supernatant fraction was injected for the direct determination of5-HT,and another part of the supernatant was allowed to react with a derivatizaˉtion reagent before the analysis of HA.Good linearity was found in the range of0.075～1.8μg/mL for HA and0.0625～1.25μg/mL for5-HT.The within-day and intra-day RSDs of the method were≤1.0%and11.4%for HA,and≤3.4%and6.3%for5-HT,respectively.The recoveries of HA and5-HT were94.1%～102.3%and72.8%～89.1%,respectively.The average contents of HA and5-HTin hypothalaˉmus of normal male Sprague-Dawley rats were0.280±0.029μg/mL and0.224±0.068μg/mL,respecˉtively.The method is simple,accurate and rapid,and can be used for the determination of HA and5-HT in rat′s hypothalamus." @default.
- W2356856787 created "2016-06-24" @default.
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- W2356856787 date "2004-01-01" @default.
- W2356856787 modified "2023-09-24" @default.
- W2356856787 title "Determination of Histamine and 5-Hydroxytryptamine in Rat′s Hypothalamus by HPLC-ECD" @default.
- W2356856787 hasPublicationYear "2004" @default.
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